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revisp v2.2 software  (SourceForge net)

 
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    SourceForge net revisp v2.2 software
    Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using <t>ReViSP</t> v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.
    Revisp V2.2 Software, supplied by SourceForge net, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revisp v2.2 software/product/SourceForge net
    Average 90 stars, based on 1 article reviews
    revisp v2.2 software - by Bioz Stars, 2026-04
    90/100 stars

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    1) Product Images from "Influence of experimental variables on spheroid attributes"

    Article Title: Influence of experimental variables on spheroid attributes

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-92037-1

    Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using ReViSP v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.
    Figure Legend Snippet: Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using ReViSP v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.

    Techniques Used: Cell Culture, Imaging, Software, Staining, Fluorescence



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    Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using <t>ReViSP</t> v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.
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    Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using <t>ReViSP</t> v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.
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    Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using <t>ReViSP</t> v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.
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    Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using <t>ReViSP</t> v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.
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    Image Search Results


    Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using ReViSP v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.

    Journal: Scientific Reports

    Article Title: Influence of experimental variables on spheroid attributes

    doi: 10.1038/s41598-025-92037-1

    Figure Lengend Snippet: Comparative analysis of HEK 293T spheroid landscapes cultured in various media. ( A ) Comparative analysis of amino acid content across different culture media. Created with ChiPlot by Songshan Zhu (https://www.chiplot.online/index.html). ( B ) Comparative analysis of amino acid formulations in frequently reported culture media for spheroid research. ( C ) Image presentation of morphology and cell death in spheroids cultured in different media types. 3D imaging revealed spatial differences in spheroid size and morphology across culture systems. 3D remodeling of spheroids was conducted using ReViSP v2.2 software (https://sourceforge.net/projects/revisp/). Scale bar = 1 mm. ( D , F ) Violin plots illustrating the influence of different culture media on spheroid metrics ( D ) and ATP content ( F ) of HEK 293T spheroids. Each biological replicate was denoted by a distinct symbol ( N ≥ 3), with each symbol representing a technical replicate ( n = 8). ( E ) Dissecting and comparing fluorescent areas of spheroid death stained with propidium iodide across different culture media. RPMI 1640 increases cell death signals compared to other media, as evidenced by elevated PI fluorescence in necrotic regions. Scale bar = 1 mm.

    Article Snippet: 3D remodeling of spheroids was conducted using ReViSP v2.2 software (https://sourceforge.net/projects/revisp/).

    Techniques: Cell Culture, Imaging, Software, Staining, Fluorescence